In-vitro Evaluation of Potential Antioxidant Properties of Eleusine indica and In-vivo Visceral Organ Protective Effect of Higher-Dose of the Phytoextract in Normotensive Rats

Abstract

The therapeutic use of natural products from indigenous plants for ethnomedicinal purposes has grown tremendous interest among scientists to search for bioactive components that are beneficial to man. This study was aimed at determining the antioxidant properties of Eleusine indica (EI) root, and as well to evaluate its effect in vivo. The in vitro antioxidant properties were estimated by standard biochemical analytical assays. For in-vivo study, the food of experimental rats were improved with the addition of EI root extract at the doses of 100 mg/kg, 200 mg/kg and 400 mg/kg for 14 experimental days, while control rat was administered a saline solution (0.9%). On day 15, experimental rats were sacrificed for histological analysis. The impact of the extract on DPPH, NO, FRAP, TAC, H2O2 radicals were dependently and ascendingly concentrated, with DPPH and NO showing maximum antioxidant capability in conjunction with IC50 values. Data obtained from the histological assay in the present study, reinforced that the compounds present in the root of Eleusine indica (EI) possesses the potential of minimizing any deleterious effects associated with higher doses of the studied plant. In this case, antioxidant and histological tests showed that the goosegrass could protect animals from damage caused by toxic agent of plant origin. This study present proof that Eleusine indica (EI) root extract remain a possible source of natural antioxidant, and that the extract of the EI at higher doses, in this study, could represent phyto-remedial indicator in protecting the rats from toxic-induced damage of plant origin, indicating that the goosegrass exhibits protective action in vivo.